Volume 8, Issue 4, July 2020, Page: 91-98
Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study
Akinrotoye Kehinde Peter, Department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Akinduti Paul, Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria
Lanlokun Olabisi, Department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Adetogun Clement, Department of Forestry and Wildlife Management, College of Environmental Resources and Management, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Received: May 25, 2020;       Accepted: Jun. 8, 2020;       Published: Jul. 6, 2020
DOI: 10.11648/j.ajbio.20200804.11      View  338      Downloads  104
Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.
Antimicrobial Resistance, MRSA, Antibiotics, Plant Extracts, Synergism
To cite this article
Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement, Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study, American Journal of BioScience. Vol. 8, No. 4, 2020, pp. 91-98. doi: 10.11648/j.ajbio.20200804.11
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